In over 190 countries all over the world, hundreds of thousands of Memmert products have been permanently in use for decades. This is why Memmert is one of the leading suppliers of temperature control appliances worldwide.
Let us take you into the fascinating world of Memmert appliances, which we have collected.
Molecular gastronomy is renowned for using laboratory equipment to create completely new dishes, flavours and textures. Copenhagen’s Alchemist is one of the top international restaurants to have applied this experimental approach as part of a unique culinary signature. In its kitchens, this innovative Danish venue has two Memmert incubators for fermenting ingredients.
Vous voulez stocker des sondes ultra-sensibles dans une enceinte climatique avec un écart de température maximal de 0,2 K? Raccourcir la durée des processus de séchage à vide? Éviter toute interruption lors de la réalisation de vos expériences à long terme? Effectuer des tests avec des taux d'échange d'air définis? Consultez dans ce cas notre page Industrie dédiée aux applications spéciales pour découvrir les appareils et les utilisations correspondant à vos besoins.
Notre engagement sans compromis en faveur de la qualité a permis à Memmert de se positionner comme un partenaire d'une grande fiabilité dans le domaine de la médecine et de la recherche médicale dès la création de l'entreprise en 1947. Inutile de préciser que la La société Memmert est d'ailleurs certifiée DIN EN ISO 13485 pour les dispositifs médicaux. Memmert a reçu la prolongation de son certificat MDD 93/42/CEE. La certification CE existante, conforme à la norme 93/42/CEE, nous a été à nouveau délivrée par l’autorité désignée et est désormais valable jusqu’à mai 2024, conformément à la disposition transitoire (UE) 2017/745. Cette page spéciale consacrée au secteur médical présente les appareils et les applications appropriés dans ce domaine.
C'est notre passion pour les détails qui assure la qualité exceptionnelle de nos appareils de contrôle thermique. C'est également l'attention minutieuse que nous portons aux attentes de nos clients qui permet aux appareils Memmert de s'imposer dans les laboratoires pharmaceutiques et ceux spécialisés dans la recherche médicale depuis plusieurs dizaines d'années. Cette page vous présente des études de cas ainsi que les appareils propres à ce domaine.
Nous avons fait du bon goût notre passion. Pour garantir la fiabilité et la précision exceptionnelles des incubateurs, étuves et enceintes climatiques Memmert, nous développons et fabriquons tous les principaux composants en interne. Découvrez ici ce que nous proposons pour l'industrie des produits alimentaires, des boissons et du tabac.
Prof. Evi Kostenis is the head of a research group at the Institute for Pharmaceutical Biology of the University of Bonn that validated a label-free screening test system for pharmaceutically active substances. It is based on optic biosensors and is applied to numerous research projects with great success. They use a Peltier-cooled incubator IPP and an incubator I, both by Memmert, to ensure precise temperature control during the assays.
With about 1000 members, the G-protein-coupled receptors (GPCRs) constitutes the largest protein family there is. Research on this family of super-proteins was honoured with two Nobel prizes, one in 1994[I] and one in 2012[II] , reflecting how important it is for intercellular communication. The exact way many physiological processes work in animal and vegetable organisms was a well-kept secret for many years and to this day, biologists, chemists and pharmacologists are still working on reproducing these molecular mechanisms as precisely as possible.
Many messenger substances such as hormones, neurotransmitters and pheromones do not enter the cells. Instead, they latch onto highly specialized receptors on the outside of the cell membrane. In the human organism, only the about 1000 different G-protein-coupled receptors[III] transmit the signals into the inside of the cells, triggering further events. Therefore, they are among the most researched target structures in pharmacology. Many pharmaceuticals such as beta blockers, antihistamines or neuroleptics are activated with GPCRs and researching further effect mechanisms opens sheer endless therapeutic possibilities. "GPCRs and their associated G-proteins are involved in many physiological effects, e.g. blood pressure regulation, airway muscle tone, cell movement, metabolism and cell proliferation." [IV]
The research group led by Prof. Kostenis at the Institute for Pharmacological Biology of the University of Bonn mainly investigates G-protein-coupled receptors and intracellular signalling pathways and therefore also focuses on determining pharmaceutically active substances that have an effect on GPCRs and the family of heterotrimeric G-proteins.
To analyse such substances, which end up both in the development of pharmaceutical products and as important tools to decode complex signalling processes, the intracellular signal transduction processes have to recorded first. This can be done by recording individual events within the cells, often using molecules died with chemical contrasting agents (e.g. with fluorescent substances) and lysing the receptor-bearing cells. However, there is always the risk that the died molecules themselves influence the interactions that are to be recorded.
For this reason, the Kostenis research group also worked with contrast agent-free analysis methods, known as label-free assays, such as dynamic mass redistribution (DMR) recording. This method records minimal changes in the optical density of the cells, as they occur when the GPCRs are activated (Schröder et.al. 2010, 2011). The DMR method is a holistic alternative to record complex signalling processes in intact living cells. What makes this method special is that it can record all four main GPCR signalling pathways, which would otherwise require an individual assay platform each. Therefore, it is possible to obtain complex, cell-based and real-time data already at an early stage of the pharmacological research process. This is the method that comes closest to in-vivo test series, potentially contributing to reducing the need for animal testing. Moreover, the DMR method is also suitable for high-throughput screening (HTS) and therefore of great interest for the pharmaceutical industry when it comes to testing libraries consisting of hundreds of thousands of substances for selected target structures.
The possibility of measuring physiological cell processes without labels and in real time - that is, without taking the detour over the reaction of luminescent or radioactive substances - and holistic recording are the main advantages modern research sees in these new screening technologies. The Institute for Pharmaceutical Biology uses an Epic® BT[V] system by Corning for label-free detection on G-proteins. The measuring system consists of a broadband light source, an optical biosensor integrated in a 384-well microplate and a detector. When polarized broadband light enters the biosensor, the light is reflected in a certain wave length. If a substance is added and the signalling pathways are activated, the distribution of the cellular mass changes, changing the wavelength of the reflected light. The measurement results show the picometre wavelength change of the reflected light, mirroring the change in optical density near the biosensor. This would typically be used to, for instance, quantify the effect of different concentrations of certain substances with concentration/effect curves (see figure).
The measurement setup of the institute includes two Memmert incubators. A small robot, which is brought to temperature in the Memmert incubator IN160, pipets the wells. DMR measurements are temperature-sensitive, therefore the temperature of the Corning Epic® BT system is brought to exactly 28 °C or 37 °C in a Peliter-cooled incubator IPP110. The incubator's cooling ability makes it possible to choose temperatures below the current room temperature on the one hand, and on the other hand allows for a faster transition between different subsequent measurements at different temperatures.
The cooled incubator IPP is especially silent and almost vibration-free, which according to Dr. Ralf Schröder and the University of Bonn was the main factor of success for the validation of the testing system. The measurements are carried out at constant temperature for about 60 to 90 minutes. Once the basal signal level has been measured, the substances used to measure the reactions have to be added - and therefore the incubator door has to be opened for about 5 seconds. In addition to the temperature stability ensured by this device, the researchers of the institute also praised the fast recovery times after opening and closing the door. To prevent radical changes in temperature, a glass inner door and a smaller special door (door in door) was installed (approx. 120 x 160 mm) that can be opened individually, keeping temperature fluctuations to a minimum.
The text of this article is largely based on information provided by the Institute for Pharmaceutical Biology of the University of Bonn. AtmoSAFE thanks Dr. Ralf Schröder and the Corning company in Wiesbaden for their friendly collaboration. The University of Bonn selected the incubators with the friendly, application-oriented advice of Th. Geyer